Study Reveals Early Molecular Signs of High-Risk Pregnancy
Women who have healthy pregnancies tend to show distinct changes in the activities of immune genes starting early in pregnancy, while women who have complicated pregnancies tend to show clear departures from that pattern, according to a new study from a team led by researchers at Weill Cornell Medicine and Hospital for Special Surgery (HSS).
The study, published April 8 in the Journal of Experimental Medicine, was designed to find early molecular predictors of the hypertension syndrome preeclampsia, miscarriage and other adverse pregnancy outcomes in women with the autoimmune disorder lupus, who face a relatively high risk of such outcomes. Over half of the more than 200 women studied were lupus patients. But the results suggest that modulation of the immune system during pregnancy is very similar in women with and without lupus.
“These findings help us understand not only pregnancy for women with lupus but pregnancy generally,” said study co-senior author Dr. Virginia Pascual, the Drukier Director of the Gale and Ira Drukier Institute for Children’s Health and the Ronay Menschel Professor of Pediatrics at Weill Cornell Medicine. Dr. Pascual has received a research grant and consulting honorarium from Sanofi-Pasteur.
“There are implications here for predicting adverse pregnancy outcomes and also for identifying therapeutic targets to prevent those outcomes,” said co-senior author Dr. Jane Salmon, a professor of medicine and of medicine in obstetrics and gynecology at Weill Cornell Medicine, and the Collette Kean Research Professor at HSS. Dr. Salmon has received an investigator-initiated grant from UCB Pharmaceuticals.
Lupus affects more than 500,000 people in the United States alone, and about 90 percent of them are women. The disorder features attacks by antibodies and other immune elements on the skin, heart, kidneys and other organs. Women with lupus who become pregnant face a roughly 20 percent chance of serious complications including miscarriage, preterm birth, stillbirth, and preeclampsia, a condition that endangers both the mother and unborn child. Prior research suggests that such complications, even in women without lupus, are at least partly caused by improper regulation of the maternal immune system—which must be tamed somewhat during pregnancy to allow tolerance of partly “foreign” fetal tissue.
From 2003 and for more than a decade afterwards, Dr. Salmon and colleagues enrolled more than 700 women, about half of them pregnant women with lupus, for a multi-center study called PROMISSE designed to uncover risk factors for adverse pregnancy outcomes. For the new study, Drs. Salmon and Pascual and their colleagues made use of blood samples and other clinical data from a subset of that cohort enrolled in 2003-13, including 92 pregnant women who had lupus and 43 pregnant women who didn’t have the disorder.
Analyses of the patterns of gene activity in these women’s white blood cells during the course of pregnancy showed that in healthy women with uncomplicated pregnancies, key elements of the immune system tended to be quieted shortly after the establishment of pregnancy, and tended to remain relatively quiet throughout the pregnancy. These were largely the same sets of genes that are overactive in lupus, and include genes relating to the production of molecules called type I interferons that marshal an immune response to viral infections.
“The surprise for us was when we looked at the data for women with lupus who had uncomplicated pregnancies,” Dr. Pascual said. “They started, as expected, with a higher level of activity in these immune pathways, but once they got pregnant these immune pathways were modulated much as they were in pregnant women without lupus.”
By contrast, in pregnant women who developed preeclampsia or other serious complications, these immune pathways were downregulated to a smaller extent from their baseline levels, or not at all.
In a separate study of 25 healthy women undergoing in vitro fertilization, the researchers observed the same healthy-pregnancy and complicated-pregnancy immune-gene signatures, and found that these patterns began to emerge at the very outset of pregnancy, with embryo implantation.
The findings, if confirmed in larger groups of pregnant women, could lead to the development of early diagnostic tests predicting pregnancy complications, and ultimately treatments that quiet specific elements of the immune system to protect women from complications. “If we could identify a pathway to target with drugs to prevent preeclampsia in lupus patients,” Dr. Salmon said, “we could immediately consider the same treatment approach to protect women who don’t have lupus but have similar high-risk biomarkers early in pregnancy."
About Weill Cornell Medicine
Weill Cornell Medicine is committed to excellence in patient care, scientific discovery and the education of future physicians in New York City and around the world. The doctors and scientists of Weill Cornell Medicine — faculty from Weill Cornell Medical College, Weill Cornell Graduate School of Medical Sciences, and Weill Cornell Physician Organization—are engaged in world-class clinical care and cutting-edge research that connect patients to the latest treatment innovations and prevention strategies. Located in the heart of the Upper East Side's scientific corridor, Weill Cornell Medicine's powerful network of collaborators extends to its parent university Cornell University; to Qatar, where Weill Cornell Medicine-Qatar offers a Cornell University medical degree; and to programs in Tanzania, Haiti, Brazil, Austria and Turkey. Weill Cornell Medicine faculty provide comprehensive patient care at NewYork-Presbyterian/Weill Cornell Medical Center, NewYork-Presbyterian Lower Manhattan Hospital, NewYork-Presbyterian Queens and NewYork-Presbyterian Brooklyn Methodist Hospital. Weill Cornell Medicine is also affiliated with Houston Methodist. For more information, visit weill.cornell.edu.
HSS is the world’s leading academic medical center focused on musculoskeletal health. At its core is Hospital for Special Surgery, nationally ranked No. 1 in orthopedics (for the 11th consecutive year), No. 4 in rheumatology by U.S. News & World Report (2020-2021), and the best pediatric orthopedic hospital in NY, NJ and CT by U.S. News & World Report “Best Children’s Hospitals” list (2021-2022). HSS is ranked world #1 in orthopedics by Newsweek (2020-2021). Founded in 1863, the Hospital has the lowest complication and readmission rates in the nation for orthopedics, and among the lowest infection rates. HSS was the first in New York State to receive Magnet Recognition for Excellence in Nursing Service from the American Nurses Credentialing Center five consecutive times. The global standard total knee replacement was developed at HSS in 1969. An affiliate of Weill Cornell Medical College, HSS has a main campus in New York City and facilities in New Jersey, Connecticut and in the Long Island and Westchester County regions of New York State, as well as in Florida. In addition to patient care, HSS leads the field in research, innovation and education. The HSS Research Institute comprises 20 laboratories and 300 staff members focused on leading the advancement of musculoskeletal health through prevention of degeneration, tissue repair and tissue regeneration. The HSS Global Innovation Institute was formed in 2016 to realize the potential of new drugs, therapeutics and devices. The HSS Education Institute is a trusted leader in advancing musculoskeletal knowledge and research for physicians, nurses, allied health professionals, academic trainees, and consumers in more than 130 countries. The institution is collaborating with medical centers and other organizations to advance the quality and value of musculoskeletal care and to make world-class HSS care more widely accessible nationally and internationally. www.hss.edu.